This essay has been submitted by a student. This is not an example of the work written by professional essay writers.

A Smart Sensor for Instantaneous Detection of Lethal Hydrazine in Human Blood and Drinking Water

  • Subject: Science
  • Category: Biology
  • Topic: Human
  • Pages 3
  • Words: 1562
  • Published: 27 March 2019
  • Downloads: 35
downloadDownload printPrint

Pssst… we can write an original essay just for you.

Any subject. Any type of essay. We’ll even meet a 3-hour deadline.

Get your price

121 writers online

Download PDF


  1. A smart chemodosimeter for Hydrazine which is highly water soluble, selective, sensitive, both colorimetric and ratiometric fluorescent probe.
  2. This probe can quantitatively detect hydrazine within 1 min.
  3. This probe showed good cell permeability, low cytotoxicity, fast fluorogenic recognition and “naked eye” detection of hydrazine below the TLV levels present in the living cells, drinking water and industrial effluent.
  4. This probe also useful to detect Hydrazine in pond and drinking water.
  5. This detects Hydrazine both in solution and solid phase with high efficiency.
  6. The 1H NMR and mass spectrometry were used to analyze the response mechanism.

Hydrazine exposure leads to damage of all vital organs of the human and animal, which demands discovery of a rapid and quantitative detection method. A newly designed and synthesized probe showed good cell permeability, low cytotoxicity, fast fluorogenic recognition and “naked eye” detection of hydrazine below the TLV levels present in the living cells, drinking water and industrial effluent.

Keywords: Chemodosimeter, Colorimetric, Rapid fluorescent sensor, Hydrazine, Bioimaging.


Hydrazine (H2N-NH2) is a chemically strong base, nucleophile and reducing agent and its high reactivity leads to wide range of application especially in the industries for large scale production of high value aerospace propellants, pharmaceuticals, agrochemicals, polymers, dyes and chemicals [1]. Flammable and detonable hydrazine is employed as an ingredient for rocket-fuels and air bag [2], syntheses of azobisformamide(AC) [3], diisopropyl carbonate and toluenesulfonhydrazide(TSH) [4], corrosion inhibitor for nuclear and electrical power plants [5], foaming polymers and alternative energy source to innovative new generation fuel cells. In spite of widespread application, hydrazine, its vapor and water solutions are explosive and poisonous in nature, which can be absorbed to humans and animals on exposure through dermal, oral and inhalation [6]. The highly toxic hydrazine reduces the average life span of the industrial workers and damages their livers, kidneys, lungs, central nervous and respiratory systems [7]. The threshold limit value (TLV) of hydrazine in drinking water must be maintained to be as low as 10 ppb [8]. Therefore instantaneous detection of extremely hazardous hydrazine has significant interest and it is especially crucial in controlling its concentration levels in the industrial area, quantitative detection in human blood and drinking water.

The qualitative and quantitative detection of hydrazine by the conventional titrimetry [9], spectrochemistry [10], Electrochemistry [11], chemiluminescence [12], chromatography [13] and surface-enhanced Raman spectroscopy [14] are not viable for in vivo analyses. In this regards, modern fluorescent techniques are simple, inexpensive, rapid and real-time monitoring, which is ideal technique for in vivo detection of hydrazine [15]. Thus, deprotection-based fluorescence sensing for hydrazine has emerged as an important tool utilizing acetyl [16-21] 4-bromo butyrate [22-23] levulinate [24-25], phthalimides [26-29], aldehydes [30-31], malononitriles [32-34], benzoic acids [35-36], γ-oxo-1-pyrenebutyrates [37], trifluoroacetylacetonates [38] and acetylacetonates [29-40]. However, most of these fluorescent probes suffers from longer detect time. Recently, Chow et al. [41] reported ethylcyanoacetate protected probe for hydrazine having detection limit 12μM and detect within 8 min. This probe shows very poor water solubility as titration was done in buffer: DMSO (1:9). Due to lack of poor water solubility these probe may not be suitable for biological application. Li et al. [42] also reported similar protected probe for hydrazine having detection limit 1.6 μM and detect within 15 min. In case of this probe, absorption and fluorescence titration was carried out in water/DMSO (4:6). Our target is to design and synthesize almost completely water soluble probe as an efficient ‘‘naked-eye’’ indicator and chemodosimeter for rapid recognition of hydrazine present in human blood and drinking water with quite low detection limit.

The benzothiazole moiety was installed in our designed probe ethyl 3-(3-(benzo[d]thiazol-2-yl)-5-bromo-2-hydroxyphenyl)-2 cyanoacrylate (3, BBHC, Scheme 1) to achieve an excited state intramolecular photon transfer (ESIPT) process upon photoexcitation [44-45]. ESIPT leads to making “O” atom in the phenolic OH highly electronegative, which in turn will induce more positive character in carbon atom of -CN. In the presence of –Br and -CN, electrophilic nature of the C=C double bond is drastically enhanced towards nucleophilic hydrazine for rapid formation of coloured hydrazone, 2-(benzo[d]thiazol-2-yl)-4-bromo-6-(hydrazonomethyl) phenol (4, BBHP).

As a result, this recognition even observable through naked eye and fluorescence change, which will be help to detect hydrazine colorimetrically and fluorogenically in a quantitative measurement.

The designed probe (BBHC) was synthesized through oxidative cyclization of o-aminothiophenol with 5-bromosalicylaldehyde (Step 1, Scheme 2) to 2-arylbenzothiazole (1), formylation of 1 to 2 (Step 2), and piperidine catalyzed condensation (Step 3) of 2 with ethyl cyanoacetate to 3. The new probe was obtained in high yields and its structure was established through 1H NMR, 13C NMR and ESI MS spectroscopic analyses (ESI).

Scheme 2: Synthetic routes to BB HC receptor (3)

Experimental section

General Methods.

All materials were purchased from Sigma-Aldrich Chemicals Private Limited and were used without further purification. All solvents were purchased from domestic suppliers and were used after distillation. 1H-NMR and 13C-NMR spectra were recorded on Brucker 300 MHz instruments. CDCl3 was used as solvent with TMS as an internal standard. Chemical shifts are expressed in δ units and coupling constants in Hz. Melting points were determined on a hot-plate melting point apparatus in an open-mouth capillary and were uncorrected. UV-vis titration experiments were performed on a Perkin Elmer Lambda 750 spectrophotometer and fluorescence experiments using Perkin Elmer LS 55 with a fluorescence cell of 10 mm path. Column chromatography was carried out by using silica gel 60 (60−120 mesh).Time-correlated single photon counting (TCSPC) set-up is used to determine fluorescence life time.

General method of UV-vis and fluorescence titrations by UV-vis and fluorescence.

A stock solution of the probe was prepared (c = 2 x 10-5 ML-1) in CH3CN: H2O (1:9, v/v). The solution of the guest anions, metal ions and amine containing compounds were prepared (2 x 10-4 ML-1) in CH3CN: H2O (1:9, v/v) at pH 7.1 by using 10 mM HEPES buffer. The solution of sensor was made ready by appropriate dilution technique. The spectra of these solutions were recorded by UV−Vis and fluorescence methods.

Details of live-cell imaging

To estimate the efficiency of BBHC to detect hydrazine endogenously 5 mL of venous blood was obtained from a healthy volunteer donor (Male, 32 year) with his informed consent. Peripheral blood mononuclear cells or PBMCs (lymphocytes and monocytes) were isolated within one hour of sampling by density gradient centrifugation using histopaque-1077 (Sigma) by centrifuging at 400 ×g for 30–40 min at room temperature. The middle layer or ‘buffy coats’ contains the PBMCs which were collected carefully and washed twice in phosphate buffered solution (PBS, pH 7.4). PBMCs were re-suspended in PBS and divided in six sets. Cells were incubated for 1 h 37 oC in dark with 10, 20, 30, 40 and 50 µM of N2H4 respectively along with 5 µM of BBHC. A separate PBMCs set was incubated simultaneously with 5 µM of BBHC but without N2H4. Intracellular fluorescence intensity was detected by fluorescence microscope (Carl Zeiss HBO 100) under 40X magnification in filter set 49 DAPI which gives emission peak for 464nm.

To determine cell viability against BBHC, PBMCs were treated with different concentrations of BBHC solution (2-20µM) for 1 h at 37 oC against control cell suspension with no added BBHC. Cell density remains 106 cells per well in a 96- well plate. 100µl of MTT solution (5mg/mL) was added to each well including control and incubated for 4 h. at 37 oC. The purple colored form zan crystals were dissolved with 100µl DMSO and the absorbance were measured at 570 nm. Cell viability was calculated using the following calculation.

Method for the preparation of TLC plate sticks.

It was easily prepared by immersing a TLC plate into the solution of BBHC (2 Χ 10-4 M) in CH3CN (1 mM) and exposing it to air to evaporate the solvent. The detection of hydrazine was carried out by inserting the TLC plate to the different concentration of hydrazine (1 mM) and evaporating solvent to dryness.


2.5.1 Synthesis of 2-(benzo[d]thiazol-2-yl)-4-bromophenol (BBP) (1):

A solution of 2- aminothiophenol (2 mL, 2 mmol) and 5 bromosalicyldehyde (400 mg, 2 mmol) in EtOH (5 mL), aqueous H2O2 (30%, 12.0 mmol) and aquous HCl (37%, 8.5 mmol) was stirred at ambient temperature for 6 h. The solution was quenched by 10 mL H2O. The precipitate was filtered, washed with ethanol, dried under vacuum and recrystallized from EtOH to afford the desired product as a white solid (480mg, 79.47% yield).

Synthesis of 3-(benzo[d]thiaol-2-yl)-5-bromo-2-hydroxybenzaldehyde (BBHB) (2):

The compound 1 (420 mg, 1.2 mmol), hexamethylenetetramine (420 mg, 3 mmol), and trifluoroacetic acid (5 mL) were added to a round bottomed flask,. The mixture was refluxed overnight. After the mixture was cooling down, the acid was neutralized with aqueous KOH solution. The precipitate was collected by filtration, and washed with water for several times. After drying under vacuum, compound 2 was obtained in >99% yield, and had the following spectral properties.

Synthesis of receptor (BBHC):

The compound 2 (100 mg, 0.3 mmol), ethylcyanoacetate (68 μL, 0.6 mmol), and 2-3 drops piperidine were dissolved in 10 mL ethanol. The mixture was refluxed for 3 h at 80 °C and cooled to room temperature. The final product (probe BBHC, 110 mg, 82%) was obtained by filtration and being washing with ethanol for three times.

Synthesis of 2-(benzo[d]thiazol-2-yl)-4-bromo-6-(hydrazonomethyl) phenol (BBHP):

BBHC was mixed with one equivalents of hydrazine in acetonitrile at room temperature to give a yellow solution. Upon removing the solvent, a solid product was obtained which was used for 1H-NMR and mass spectroscopy.

Remember: This is just a sample from a fellow student.

Your time is important. Let us write you an essay from scratch

experts 450+ experts on 30 subjects ready to help you just now

delivery Starting from 3 hours delivery

Find Free Essays

We provide you with original essay samples, perfect formatting and styling

Cite this Essay

To export a reference to this article please select a referencing style below:

A smart sensor for instantaneous detection of lethal hydrazine in human blood and drinking water. (2019, March 27). GradesFixer. Retrieved November 30, 2021, from
“A smart sensor for instantaneous detection of lethal hydrazine in human blood and drinking water.” GradesFixer, 27 Mar. 2019,
A smart sensor for instantaneous detection of lethal hydrazine in human blood and drinking water. [online]. Available at: <> [Accessed 30 Nov. 2021].
A smart sensor for instantaneous detection of lethal hydrazine in human blood and drinking water [Internet]. GradesFixer. 2019 Mar 27 [cited 2021 Nov 30]. Available from:
copy to clipboard

Sorry, copying is not allowed on our website. If you’d like this or any other sample, we’ll happily email it to you.

    By clicking “Send”, you agree to our Terms of service and Privacy statement. We will occasionally send you account related emails.


    Attention! This essay is not unique. You can get a 100% Plagiarism-FREE one in 30 sec

    Receive a 100% plagiarism-free essay on your email just for $4.99
    get unique paper
    *Public papers are open and may contain not unique content
    download public sample

    Sorry, we could not paraphrase this essay. Our professional writers can rewrite it and get you a unique paper.



    Please check your inbox.

    Want us to write one just for you? We can custom edit this essay into an original, 100% plagiarism free essay.

    thanks-icon Order now

    Hi there!

    Are you interested in getting a customized paper?

    Check it out!
    Having trouble finding the perfect essay? We’ve got you covered. Hire a writer

    Haven't found the right essay?

    Get an expert to write you the one you need!


    Professional writers and researchers


    Sources and citation are provided


    3 hour delivery