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In week one lab, we took brain heart agar and thioglyollate broth and used them to determine the used bacteria’s oxygen requirements using 8 different bacteria. We also used 2 TSA plate with 8 different parts to test the oxygen requirements of Clostridiam Sporogenes, E. coil, Micrococcus lateus, B. cereus, Staph. Aureus, Corynebacteriam xerosis, Enterococcus Faerolis and S. cerevisiae. One plate will be placed in the gas pack chamber, and the other will be incubated. We suspect that we will see more oxygen dependent growth with E. coil, Staph. Aureus, Enterococcus Faerolis, and Clostridiam Sporogenes.
In week two lab, we took 5 different bacteria and placed them on Mueller hinton plates to grow. Using the bacteria E.coli, Staph aureus, protest Vulgaris, S. cerevisiase, and enterococcus faecelis. In growing these bacteria, we will also be using antibacterial disks to see the bacteria’s reaction to the antibacterial, and rate its “halo” or reaction. The antibiotics used in this experiment are cephalothin, cefazonlin 30, azithromycin, sulpha/trimeth, and tetracycline.
In week three lab, we had 6 different bacteria, and four different medias to determine the species or the bacteria, and to test for which sugar each bacteria eats. Alcaligenes faecalis, E. coli, Micrococcus luteus, Staph. Aureus, Shigella Flexneri, and Proteus vulgaris were the bacteria used in this experiment. Lactose, sucrose, dextrose, and TSI were the sugars that were being tested. The agar TSI and broths will change color when a bacteria likes a sugar, or stay red if the bacteria is in a solution that is not compatible to its surroundings. We hypothesis that Micrococcus luteus, Staph. Aureus, Shigella Flexneri, and Proteus vulgaris will like lactose, E. coli, Staph. Aureus, Shigella Flexneri, and Proteus vulgaris will like sucrose, and all bacteria will like dextrose.
In week four, we are testing the different parts of our body for infections. Using streak plates, blood agar and MSA, we will see growth on both plates. We are going to test our face, behind the ear, corner of eye, and inside the nose. We will see gram positive growth on MSA and blood for all swabs. On our face and behind the ear, we will also see a color change on the MSA plate because they are more oil prone.
Materials needed for this lab includes: Clostridiam Sporogenes, E. coil, Micrococcus lateus, B. cereus, Staph. Aureus, Corynebacteriam xerosis, Enterococcus Faerolis, S. cerevisiae, Alcaligenes faecalis, Micrococcus luteus, Shigella Flexneri, TSI, sucrose, lactose, dextrose, TSA plate, incubator, tubes, swabs, lab coat, hockey sticks,
For week one lab, we used a needle and a loop to transfer the 8 bacteria to the brain heart agar, thioglyollate, and the two TSA plates. Using the needle, we used the broth to slant transfer for all 8 bacteria using the needle. The needle with the bacteria was placed in the brain heart agar straight down in the middle and not all the way down to the bottom. Using the loop, we conducted the broth to broth transfer with the thioglyollate with all 8 bacteria. Using the loop, we also did one streak on each designated area for the TSA plate.
For week two lab, we used a ‘hockey stick’ to spread the 5 bacteria around the 5 individual plates. After the bacteria were placed on the Muller Hinton plates, they were separated in 5 sections, and placed the chosen antibiotics in the said sections. The plates were placed in the incubator for two days.
In week three, we had Alcaligenes faecalis, E. coli, Micrococcus luteus, Staph. Aureus, Shigella Flexneri, and Proteus vulgaris used as our bacteria. Using loop to broth transfers, we transferred the bacteria to sucrose, lactose, dextrose, and with needle to agar transfer to TSI. All the tubes were labeled and placed in the incubator for 2 days. Within the fermentation of this experiment, we will also look at gasses being released within the solutions, and the presence of acid production, and sulfur.
In week four, we used MSA and blood plate agars to test the 4 parts of our head. We swabbed our face, behind the ear, corner of the eye, and inside our nose. Using 2 plates of each, we separated each plate into 6 places and used the swabs to place in the designated areas of the agars. The agars were incubated for two days.
In week one, Clostridiam Sporogenes was the only bacteria that had no growth which could have been in result of no bacteria to begin with. E. coli, B. cereus, and Enterococcus Faerolis had the most growth in the tubes, and growth in both plates. Corynebacteriam xerosis had growth in the tubes, but when placed in the plates, it had no growth. Everything but Micrococcus lateus and Corynebacteriam xerosis, which had aerobic growth, had napiform growth on the brain heart agar. Micrococcus lateus, Staph. Aureus, Corynebacteriam xerosis, and S. cerevisiae are aerobic which means that there reliant on oxygen, and need it to survive. E. coli, Staph. Aureus, and Enterococcus Faerolis are areo tolerant which means that they can live in low oxygen environments, but they thrive without it. B. cereus, Staph. Aureus, Enterococcus Faeroli, and S. cerevisiae are microaerophilic and need oxygen for fermentation, but do not survive on the top of the broth. In regards of our hypothesis, we were correct about the bacteria needing more oxygen, but did not have all of the bacteria listed that were aerobic. In doing this experiment again, I would like to see how temperature affects the growth of the bacteria and if they would still be as dependent, or non-dependent on oxygen.
In week two, we found that with S. cerevisiase, yeast, antibiotics do not have a large effect on destroying the cells. E.coli was best treated with sulphamethozole and trimethoprim which are broad spectrum treatments. S. aureus was treated by all 5 antibiotics, but cefazolin was more effective than the others. Protest Vulgaris had two high treatment doses of sulphamethozole and trimethoprim, as well as tetracycline. Enterococcus faecelis was the second hardest to penetrate, but Cefazolin turned out to be the most effective on the bacteria. Over all, sulphamethozole and trimethoprim had the highest doses with the exeption of S. cerevisiase and Enterococcus faecelis which had no effect on the bacteria. In doing this experiment again, I would have viruses as well as bacterial infections to see how viruses react to the antibiotics.
In week three, all bacteria were positive for glucose in this experiment. Alcaligenes faecalis does not eat sucrose or lactose, but it does metabolize dextrose. E. coli does not like sucrose, but did metabolize lactose and dextrose, but was negative in the TSI for sucrose and lactose. E. coli also gave off gasses in lactose and dextrose, but only in the dextrose for TSI. A reason that the TSI test for sucrose and lactose was negative is because if one is negative, then that’s what is visible. Micrococcus luteus was positive for sucrose and dextrose, but negative for lactose. TSI agar showed that Micrococcus luteus was negative for sucrose and lactose, but positive for glucose. Gas was present in all sugars. Staph. Aureus was positive for all sugars, and gas was present in everything but dextrose. Shigella Flexneri was positive for all single sugars, but negative for lactose and sucrose in the TSI agar. Gas was present in lactose and dextrose. Proteus vulgaris was positive for all single sugars, but negative for lactose and sucrose in the TSI agar, and Hydrogen sulfide was present in the TSI agar. In doing this experiment again, I would like to add different types of pH level acids to see what bacterium can survive in certain pH levels.
In week four, we found that the blood agar tested positive for bacteria on all tests. The oil and behind the ear test had significant growth for all precipitants. Inside the nose had significant growth for Courtney and Audrey, but Shelby did not show as significant growth. Courtney and Shelby had little growth on their eye swab, but Audrey had significant growth on her swab. The different results can be based off of how much bacteria was on each swab. Shelby and Courtney also wear contacts which could have skewed the results. Growth on MSA plates demonstrates the growth of Staphylococcus. The oil test, swabbing of the T- zone on face, and the swab behind the ear were the most significant because of the oil content. Courtney and Shelby had no growth from their nose or the corner of their eye swabs, but Audrey had maximal growth on the nose swab, and some growth on the eye swab. There were four different types of bacteria growing total. Audrey said that she hasn’t been feeling well lately and has been experiencing cold like symptoms that could have affected her results. In continuing investigation, Audrey and Shelby was their face on a daily basis whereas Courtney does not. Audrey and Courtney wear foundation where Shelby only wears mascara. In doing this experiment again, I would have the people wearing no makeup and test the same area the next day and examine the results.
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