Acridone Influence On Hepatoccellular Carcinoma (Hcc): [Essay Example], 2142 words GradesFixer
exit-popup-close

Haven't found the right essay?

Get an expert to write your essay!

exit-popup-print

Professional writers and researchers

exit-popup-quotes

Sources and citation are provided

exit-popup-clock

3 hour delivery

exit-popup-persone
close
This essay has been submitted by a student. This is not an example of the work written by professional essay writers.

Acridone Influence on Hepatoccellular Carcinoma (hcc)

Download Print

Pssst… we can write an original essay just for you.

Any subject. Any type of essay.

We’ll even meet a 3-hour deadline.

Get your price

121 writers online

blank-ico
Download PDF

INTRODUCTION:

Hepatoccellular carcinoma (HCC) is a global health problem with approximately around 8 lakh death per year, moreover there exist a very limited treatment options. Surgery remains to be the most effective way for the suppression of HCC but it is associated with sever other problem of relapse and distant organ invasion after surgery. So to get rid of such invasion and relapse a chemotherapeutic agent is highly recommended that can clear the survived tumor cells post dissection. Therefore, choosing appropriate anti-tumor agents and planning their administration is an indispensable part of systemic treatment HCC. Acridone derivatives are one such novel chemotherapeutic agent that can be explored for the treatment of HCC.

Acridone is a biologically active fused heterocyclic rings containing compound known to be associate with several biological activities. It has carbonyl group at 9th position and nitrogen at 10th position and is an oxidized product of acridine. Acridone is also known by the name of 9(10H)-acridinone, acridine-9-one, 9-acridanone, acridine 9, 10-dihydro-9-oxo, 9, 10-dihydro-9-oxo acridine, acridinone and 9-azanthracene-10-one. Its derivatives are known for its chemotherapeutic agents that were initially antibacterial and antiparastic agents. The biology of acridines is mainly attributed to the planarity of these aromatic structures which can intercalate within the double –stranded DNA structure, thus interfering with nuclear machinery. In view of the above facts, it is of considerable interest to synthesize the titled compounds with a hope to obtain potent anticancer agents. A survey of chemical literature has discovered that the acridone ring nucleus substituted at N10 position with tertiary amino groups (N-methylpiperazino, piperidino, morpholidino, diethylamino, diethanolamino and (β – hydroxyethyl) piperizine) at a distance of 3 to 4 carbon reported to posses anticancer activity. Moreover compound with anticancer agents are reported to have a potent antioxidant property. With an intention of discovering a better anticancer agent and antioxidant a series of possible compounds have been prepared on, N10 acridones by Ulmann’s condensation method.

Materials and Methods:

Synthesis

Preparation of 3-Chloro-4-Fluoro- Diphenylamine-2-Carboxylic acid:

To a mixture of o-chlorobenzoic acid (A) (5g, 0.032 mmole), 0-3 chloro, 4-fluoro aniline (5g 0.046 mmoles) and copper powder ( 0.2 g) in 30ml isoamyl alcohol, dry potassium carbonate (10 g ) was slowly added and the contents were allowed to reflux for 6 hours on an oil bath. Precipitate formed was filtered, washed with hot water and collected. The crude acid was dissolved in aqueous Sodium hydroxide solution, boiled in the presence of activated charcoal and filtered. On acidification of the filtrate with concentrated hydrochloride acid, light Yellowish precipitate was obtained which was washed with hot water and recrystallized from ethanol to give light yellow solid (yield 72%, mp 188 0C). IR Spectrum (cm-1) 3055 (OH), 2420 (NH), 1639 (C=O), 1097 (C-F), and 846 (C-Cl).

Cyclisation of 3-Chloro-4-Flouro-diphenylamine to 3-Chloro-4- Fluoro Acridone

Six grams of amino benzoic acid was taken in a round bottom flask to which was added 60 g of polyphosphoric acid. Shaken well and heated on a water bath at 100 0C for 3 hours. Appearance of yellow colour indicated the completion of the reaction. Then, it was poured into one liter of hot water and made alkaline by liquor ammonia. The yellow precipitate that formed was filtered, washed with hot water and collected. The sample of 3- Chloro, 4- Fluoro acridone (1) was recrystallized from acetic acid (yield 76%, mp 323 0C). Further, Purity of the compound was checked by TLC (chloroform: methanol = 24:1) and the purified product characterized by spectral methods. IR Spectrum (cm-1) 2970 (NH), 1685 (C=O), 1087 (C-F), and 763(C-Cl). 1H NMR (CDCl3- d6) δ: 10.8(S, 1H, NH), 7.2-8.6(m, 6H,Ar-H)

Synthesis of N10 – alkylated Acridones via Phase Transfer Catalysis.

10-(3’-N-Chloropropyl] – 3-Chloro- 4 -Fluoro acridone .

One gram (0.0047 mmole) of 3-Chloro-4- Flouro acridone was dissolved in 20ml tetrahydrofuran and added 25ml of 6N potassium hydroxide and 0.74g (2.30 mmole) tetrabutylammonium bromide to it. The reaction mixture was stirred at room temperature for half an hour and added 1- bromo -3-chlroprophane (0.69 mmole) slowly into the reaction mixture stirred for 24 hours at room temperature. Tetrahydrofuran was evaporated and the aqueous layer was extracted with chloroform. The chloroform layer was washed with water and organic layer dried over anhydrous sodium sulphate and rotaevaporated. The crude product was purified by column chromatography by using the solvent system chloroform – acetone (8:1) to give yellow solid of 10-(3’-N-Chloroprophyl)-3-Chloro-4-Flouro acridone (2) (yield 38%, mp 108 0C). IR Spectrum (cm-1) 2829 (Ar,C-H), 1687 (C=0), 1085 (C-F), and 758 (C-Cl). 1HNMR (CDCl3- d6)δ: 7.2-8.0(m, 6H, Ar-H), 1.25-1.9(m, 6H, Hk, Hl and Hm)

Synthesis of N10Chloropropyl- 3-Chloro-4-Fluoro acridone derivatives:

(Piperidine/N-Methyl /Morpholine/Pyridine/Diethylamine/Diethanolamine)

One gram (0.0034 mmole)of 10-(3-N-chloroprophyl)-3-Chloro-4-Fluoro acridone was dissolved in 30ml of anhydrous acetonitrile and 1.13g potassium iodide and 2.18g of potassium carbonate were added and refluxed for 30 minutes . Then added 1.10g (0.34mmole, 1.22ml) of various secondary amines into it slowly and refluxed for 15 hours until a substantial amount of the product was formed as evidenced by TLC. The contents were cooled, diluted with water and extracted with chloroform; the chloroform layer was washed with water thrice, dried over anhydrous sodium sulphate and evaporated to give an oily product. The oily residue was purified by column chromatography using the solvent system chloroform- acetone (8:1) to give a light yellow oil of 10-[3-(N- Respective-propyl)-3–Chloro-4-Flouro] acridone derivatives. An acetone solution of the free base was treated with ethereal hydrochloride to give the hydrochloride salt that was dried over high vacuum to get pure solids of various yields and melting points. IR Spectrum (cm-1) 3435 (OH), 1626 (C=O), 1047 (C-F), and 812 (C-Cl). 1HNMR (CDCl3- d6)δ: 7.2-8.4(m, 6H, Ar-H), 2.9-3.7(m, 6H, Ha, Hb, and Hm) and 1.70-1.8(m, 6H, Hc, Hd and Hc) and 1.2-1.4(m, 4H, Hk and Hl).

Scheme 1

Anticancer activity

Lung Cancer (HepG2) cell line

Lung cancer cell line (HepG2) cell line was procured from Sigma Aldrich Chemicals Pvt Ltd, Bangalore and maintained in Minimum Essential Medium Eagle Medium (EMEM) with 2 mM Glutamine, 1% Non-essential amino acid and 10% Foetal Bovine Serum (FBS), and antibiotic mixture (Penicillin, streptomycin and ampicillin 100 units/ mL) under defined conditions of temperature at 37oC, 95% humidity and 5% CO2.

Cytotoxicity Assay

The cytotoxic activity of the different newly synthesized compounds was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay. [14] Sorafanib is taken as the standard control. Each chemical was dissolved in phosphate buffer saline (PBS) buffer, sterilized by membrane filtration, and then serially diluted with culture medium to different concentrations. Lung cancer cell line (HepG2) was trypsinised and the cells were counted using haemocytometer following standard procedure. 100µl of the lung cancer cell line at 1 X 104 cells/mL was added to poly 96 well plate and incubated at 37 oC in a humidified 5% CO2 incubator. After 24 hours of incubation, the old medium was replaced with fresh medium and 50 µL of the test compounds was added and incubated for 48 hours at 37 oC in a humidified 5% CO2 incubator. 30µl of 0.5% w/v MTT was added and incubated at room temperature for 4 hours. After incubation, 50 µL of acid-isopropanol was added to dissolve the formazan formed and incubated at room temperature for 30 minutes. Then absorbance was taken at 554nm using Bio-Rad micro-titer plate reader. The IC50 values were derived using ELISA software (Gen 5.0 Secure). Later, morphology of the cells was observed by phase contrast microscopy using Nikon Eclipse TS100.

Antioxidant assay

DPPH assay

The antioxidant assay was performed using the free radical scavenging activity of (2, 2-diphenyl-1-picryl-hydrazyl-hydrate). About 1 mg of the synthesised compound was dissolved in 1ml of methanol. About 10 mL of 0.1 mM of DPPH was prepared in methanol and stored in cool dark condition until use. Accurately, 1 mL of DPPH was added to different concentration of the synthesised compound. The mixture of DPPH and synthesised compound was incubated at room temperature for 30 minutes in dark, and then the absorbance was measured at 517 nm in UV spectrophotometer. Ascorbic acid was used as a reference and DPPH without the synthesised compound served as negative control. The IC50 value of the sample was calculated based on the absorbance. The percentage of inhibition was calculated using the formula,

DPPH scavenging activity (%) = [1- (Asample/Acontrol)] x 100

Where, Asample and Acontrol are the absorbace of the sample and the control respectively.

H2O2 scavanging activity:

30mg of the synthesized compounds and the standard were dissolved separately in 30 ml DMSO to make a stock solution of 1 mg/ml. From the above solutions further dilutions were made to get different concentrations. Taken 1 ml of each dilution of synthesized compounds and standard drug and then added 2 ml H2O2 in PBS. The absorbance of hydrogen peroxide at 230 nm was determined after ten minutes against a blank solution containing phosphate buffer saline without hydrogen peroxide. The percentage of hydrogen peroxide scavenging by the test compounds and standard drug was calculated as follow:

% Scavenged [H2O2] = [(A0-At)/ A0] x 100

Where A0 was the absorbance of the control and At was the absorbance in the presence of the sample and standard. Calculated the IC50 values by the graph were plotted between % scavenging on Y axis and concentration on X axis.

Statistical Analysis:

All the datas are expressed as Mean ± SEM of the treatments and are analysed by oneway analysis of variance. Dunnett multiple comparison post test were performed with significant value set at p<0.05.

Results and Discussion:

The N10 substituted acridones compounds were synthesized according to the procedures as given in the Scheme. The reactions were monitored by TLC. The Physiochemical properties like melting point and solubility were determined for all the intermediate and final products. The compounds were further characterized by IR, and 1H NMR. All the titled compounds were evaluated for in-vitro cytotoxic activity, antioxidant activity.

Cytotoxic studies:

The cytotoxic studies were performed in HepG2 cell lines. The results for cytotoxic studies are shown in table 1. It Indicates the IC50 value of synthesized acridone derivatives by in- vitro cytotoxic activity. The synthesized compounds were screened for in-vitro cytotoxic activity by MTT assay (Microculture tetrazolium) using HepG2 cell line. Sorafenib was used as a strandard. Data was analysed and the IC50 values were derived using ELISA software (Gen 5.0 Secure). According to IC50 values all the compound showed significant cytotoxic activity at different dose.

The observations suggested that the compounds synthesized has a significantly less dose as IC50 (p<0.01) as ccompared to the standard sorafenib.

Table 1: IC50 value of synthesized acridone derivatives by in- vitro cytotoxic activity.

SL. No. Compound and Mol. Formula IC50 (mg/L)

Control – –

C22H26N2O.HCl 0.021 ± 0.005**

C22H27N3O.HCl 0.015 ±0.0061 **

C21H24N2O2.HCl 0.081 ± 0.0012**

C21H24N2O.HCl 0.078 ±0.0042 **

C21H26N2O.HCl 0.091 ± 0.0062**

C21H26N2O3 0.097 ± 0.0072**

Standard Sorafenib (C21H16ClF3N4O3 ) 2.7 ± 0.010

Data was analysed and the IC50 values were derived using ELISA software (Gen 5.0 Secure)

The morphological studies by phase contrast microscopy further confirms that the compound treated with the synthesized compounds are slow growing, round shaped, indistinct and detached from the bottom of the plate. These results suggested that after 24 h of the treatment with the synthesized compound significantly reduced the growth of HepG2 cells. The cytotoxic activity of the compounds as suggested by Fukui et al may be due to the planar structure of the acridones that binds directly to the DNA.

Antioxidant activity

Further to see the effect of the synthesized compound on antioxidant activity DPPH and H2O2 scavenging activity was performed using Ascorbic acid as a standard drug. The graphs were plotted between concentration and % inhibition to calculated the IC50 value. Table No. 2 showed the effect of synthesized acridone derivative on in-vitro antioxidant activity by DPPH and H2O2 scavenging method.

Table 2: DPPH and H2O2 scavanging activity of synthetic derivatives.

molecular formula DPPH free radical scavanging activity H2O2 scavanging activity

% Inhibition IC50 (mg/ml) % Inhibition IC50 (mg/ml)

1 C22H26N2O.HCl 0.53 – 78.3 124 ± 1.6*** 0.53 – 78.3 124 ± 1.6***

2 C22H27N3O.HCl 0.46 – 64.7 365 ± 0.67*** 0.46 – 64.7 365 ± 0.67***

3 C21H24N2O2.HCl 2.2 – 92.7 155 ± 0.86*** 2.2 – 92.7 155 ± 0.86***

4 C21H24N2O.HCl 0.66- 47.3 345 ± 1.86*** 0.66- 47.3 345 ± 1.86***

5 C21H26N2O.HCl

0.66 – 47.3 > 440 0.66 – 47.3 > 440

6 C21H26N2O3 1.78 – 49.1 >470 1.78 – 49.1 >470

Standard Ascorbic Acid 45 – 98 13.33 ± 0.88 45 – 98 13.33 ± 0.88

The results are expressed as mean ±SEM

The result of antioxidant activity suggested that IC50 of all the compounds are highly significant as compared to the standard Ascorbic acid (p<0.01) inferring that the antioxidant property of the synthesized compounds lies on significantly high dose. Another observation from its antioxidant property is that the compounds having substitutes of heterocylic ring system on 10-[3’- (N-substituted butyl)-3-chloro-4-fluoro]-acridones (ie compound 1, 2, 3 and 4) has shown better antioxidant activity whereas compounds having substitutes of long alkyl chain (ccompound 5 and 6) has no antioxidant property. This may be due to the ability of heterocyclic moiety to quench the free radical generated due to the electron donation or it acts as a reducing agent in the ring making it comparatively better than long chain alkyl groups which are electron deficient.

Remember: This is just a sample from a fellow student.

Your time is important. Let us write you an essay from scratch

100% plagiarism free

Sources and citations are provided

Find Free Essays

We provide you with original essay samples, perfect formatting and styling

Cite this Essay

To export a reference to this article please select a referencing style below:

Acridone Influence On Hepatoccellular Carcinoma (Hcc). (2019, August 08). GradesFixer. Retrieved October 27, 2020, from https://gradesfixer.com/free-essay-examples/acridone-influence-on-hepatoccellular-carcinoma-hcc/
“Acridone Influence On Hepatoccellular Carcinoma (Hcc).” GradesFixer, 08 Aug. 2019, gradesfixer.com/free-essay-examples/acridone-influence-on-hepatoccellular-carcinoma-hcc/
Acridone Influence On Hepatoccellular Carcinoma (Hcc). [online]. Available at: <https://gradesfixer.com/free-essay-examples/acridone-influence-on-hepatoccellular-carcinoma-hcc/> [Accessed 27 Oct. 2020].
Acridone Influence On Hepatoccellular Carcinoma (Hcc) [Internet]. GradesFixer. 2019 Aug 08 [cited 2020 Oct 27]. Available from: https://gradesfixer.com/free-essay-examples/acridone-influence-on-hepatoccellular-carcinoma-hcc/
copy to clipboard
close

Sorry, copying is not allowed on our website. If you’d like this or any other sample, we’ll happily email it to you.

    By clicking “Send”, you agree to our Terms of service and Privacy statement. We will occasionally send you account related emails.

    close

    Attention! this essay is not unique. You can get 100% plagiarism FREE essay in 30sec

    Recieve 100% plagiarism-Free paper just for 4.99$ on email
    get unique paper
    *Public papers are open and may contain not unique content
    download public sample
    close

    Sorry, we cannot unicalize this essay. You can order Unique paper and our professionals Rewrite it for you

    close

    Thanks!

    Your essay sample has been sent.

    Want us to write one just for you? We can custom edit this essay into an original, 100% plagiarism free essay.

    thanks-icon Order now
    boy

    Hi there!

    Are you interested in getting a customized paper?

    Check it out!
    Having trouble finding the perfect essay? We’ve got you covered. Hire a writer

    GradesFixer.com uses cookies. By continuing we’ll assume you board with our cookie policy.