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An Experiment Conducted in Attempt to Transform DNA off the Bacteria E. Coli

Abstract: The main objective of this experiment was to transform DNA found in bacteria. A plasmid was used on E. coli and with the use of heat shock, was inherited by the bacteria which caused the E. coli to become resistant to ampicillin. Also in...

E Coli 2 Pages

The Growth Rate (k) and Generation Time (g) of the E. Coli Cultures in Terms of Different Temperatures: A Lab Study

Microbial Growth In this lab we measured the absorbance, at 15 minute intervals, of Escherichia coli in tryptic soy broth using a spectrophotometer. Using measurements of optical density (OD) provided by the spectrophotometer we are then able to calculate the values for growth rate (k)...

E Coli 2 Pages

An Experiment to Change E. Coli Genetically to Express Green Fluorescent Protein with the Use of Plasmids

Use of Plasmids to Genetically Transform E. Coli to Express Green Fluorescent Protein Abstract In this lab, we tested gene transformation by inserting pGLO plasmid into E. Coli cells to express the Green Fluorescent Protein. We predicted that only transformed E. Coli would grow in...

E Coli 4 Pages