Types of Biochemical Analysis Techniques

Biochemical analysis techniques submit to a set of methods, assays, and procedures that allow scientists to analyze the substances establish in living organisms and the chemical reactions primary life processes. The most stylish of these techniques are reserved for specialty research and diagnostic laboratories, although simplified sets of these techniques are used in such common events as testing for prohibited drug abuse in competitive athletic measures and monitoring of blood sugar by diabetic patients.

Biochemical analysis is a fast increasing field and is a key part of modern drug discovery and research. To achieve a comprehensive biochemical analysis of a biomolecule in a biological process or system, the biochemist typically wants to design a plan to take in that biomolecule, separate it in pure form from among thousands of molecules that can be found in an extract from a biological sample, characterize it, and analyze its purpose. An assay, the biochemical test that characterize a molecule, whether quantitative or semi-quantitative, is important to determine the presence and quantity of a biomolecule at each step of the study. Detection assays may series from the simple type of assays provided by spectrophotometric measurements and gel staining to terminate the concentration and clarity of proteins and nucleic acids, to long and tedious bioassays that may get days to achieve

Different types of techniques

• • Electrophoresis

Electrophoresis techniques take benefit of both the size and charge of biomolecules and submit to the process where biomolecules are separated because they adopt dissimilar rates of migration toward positively (anode) or negatively (cathode) emotional poles of an electric field. Gel electrophoresis methods are important steps in many separation and analysis techniques in the studies of DNA, proteins and lipids.

• • Centrifugation

Centrifugation actions impose, through rapid spinning, high centrifugal forces on biomolecules in solution, and cause their separation based on differences in weight.

• Chromatography

Chromatography techniques are sensitive and effective in separating and concentrating tiny components of a mixture and are generally used for quantitative and qualitative analysis in medicine, industrial processes, and other fields.

• • Spectrophotometry

This techniques offer knowledge of the biomolecules such as proteins, lipids, carbohydrates, nucleic acids and their functions.

A spectrophotometer is an instrument that actions the amount of photons (the intensity of light) immersed after it passes through sample solution. With the spectrophotometer, the quantity of a known chemical substance (concentrations) can also be determined by measuring the intensity of light detected.

• • ELISA

Molecular and immunological techniques such as ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning protocol dealt in this way are mainly focused on understanding the diagnosis of diseases, malfunctions, and disorders in order to generate corrective measures.

In recent years, huge advance are made in understanding the gene structure and their expression and hence, the discipline of molecular biology overlaps with that of biochemistry in many aspects. Molecular and immunological techniques such as ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning protocol dealt in this course are mainly focused on understanding the diagnosis of diseases, malfunctions, and disorders in order to generate corrective measures.

• • X-ray microscope

An X-ray microscope uses electromagnetic radiation in the soft X-ray band to produce magnified images of objects. Since X-rays enter most objects, there is no want to specially practice them for X-ray microscopy observations.