close
test_template

Vesiculobullous Disorders

download print

About this sample

About this sample

close

Words: 668 |

Page: 1|

4 min read

Published: Sep 25, 2018

Words: 668|Page: 1|4 min read

Published: Sep 25, 2018

Table of contents

  1. Objectives
  2. Materials and Methods
  3. Implications of Study
  4. References

Vesiculobullous disorders represent a heterogenous group of dermatoses with protean manifestations Vesicles and Bullae are fluid filled cavities formed within or beneath the epidermis. In this study, skin punch biopsy is used for diagnosis. Punch biopsy is a simple, inexpensive, safe OPD procedure, causing minimal discomfort to the patient and no scarring. These disorders have remarkable impact on the patient and their family, and have severe economic consequences.

Pathologic evaluation of blisters involves systematic analysis, which includes the blister separation plane, the mechanism of blister formation and the character of the inflammatory infiltrate, including its presence or absence. Recent advances in investigative dermatology have created new horizons. The most important techniques for the investigation of patients with Vesiculobullous disease are conventional histopathology and confirmative tests like direct and indirect immunofluorescence

Immunofluorescence has a very important role in the diagnosis, treatment and understanding of the pathophysiology of Vesiculobullous lesions of skin. It is also an important prognostic tool, as positive direct immunofluorescence (DIF) findings in patients in remission predict early relapse of disease Research techniques such as immunoblotting and immunoelectron microscopy may refine the diagnosis in the individual patient. However, these investigations are available only in advanced research laboratories. DIF is also done only in a few centres in developing countries like India. With the availability of transport media like Michel’s media, majority of dermatologists can have access to DIF.Therefore,this study is an attempt to evaluate Vesiculobullous lesions using both histopathology and direct IF (DIF) studies and to assess the diagnostic potential of the IF studies.

Objectives

  1. To study histopathological changes by light microscopy in Vesiculobullous disorders of the skin and to correlate clinical diagnosis and histopathological diagnosis of Vesiculobullous disorders of the skin.
  2. To correlate the histopathological diagnosis with immunofluorescence findings and determine the utility of Direct immunofluorescence in the diagnosis of Vesiculobullous disorders.
  3. To evaluate the clinical diagnosis of Vesiculobullous lesions and correlate it with histopathological findings and the findings from immunofluorescence

Materials and Methods

  • Study duration:4 months
  • Place of study: Ramaiah Medical College
  • Study design:Retrospective cross sectional study
  • Inclusioncriteria: Patients clinically diagnosed with active Vesiculobullous diseases irrespective of age and sex.
  • Exclusion criteria:
    1. Patients with no active lesions
    2. Patients on systemic steroids
    3. Patients on immunosuppressive therapy.
  • Procedure and Materials required: Biopsy for Direct immunofluorescence was taken from the perilesional skin. On DIF examination, the following parameters were evaluated:
    1. Site of deposition of immunoreactants (Epidermis/Basement membrane zone/dermis)
    2. Pattern of immunofluorescence(Linear/Granular) Intensity ofimmunofluorescence- graded as +++ strongly positive, ++ Moderately positive and Negative.
  • Statistical Analysis
    1. Sample size: 68
    2. Rationale for sample size: Based on the literature review,in previous studies it was found that Vesiculobullous lesions constitutes 22.08% of total number of skin biopsies.

The most common being Pemphigus vulgaris followed by bullous pemphigoid, pemphigus foliaceous and dermatitis herpetiformis. In a present study expecting similar proportions considering absolute precision of 4% to achieve a desired confidence level of 95% the sample size worked out to 68 cases of Vesiculobullous lesions.

Get a custom paper now from our expert writers.

Implications of Study

Through this study we aim to categorize Vesiculobullous lesions based on their response to IF. We aim to find a quicker and accurate diagnosis for Vesiculobullous skin lesions that are difficult to diagnose on the basis of clinical and histopathological features. So, in this study we corelate and evaluate the clinical findings of Vesiculobullous lesions with positive or negative results of histopathological and IF evaluation.

References

  1. Rajeswari Thivya Dhanabalan et al,The utility of immunofluorescence in diagnosing dermatological lesions and its correlation with clinical and histopathological diagnosis in a tertiary health care setup,Indian Journal of Dermatopathology and Diagnostic Dermatology. 2016: 63-70
  2. Rohit Bhalara et al. A histopathological study of Vesiculobullous lesions of skin, Annals of Pathology and Laboratory Medicine, 2016; 3: 7-9
  3. Prashant R Patel et al. Histopathologicalstudy of Vesiculobullous Lesions ofthe skin: A Study at Tertiary Care Hospital, International Journal of Medical Science and Public Health , 2014;3 :738-740
  4. Huilgol SC , Bhogal BS, Black MM. “Immunofluorescence of immunobullous disorders part one : methodology”’ Indian J Dermatol Veneral Leprol. 1995; 61; 187-95
  5. Inchara YK, Rajalakshmi T. Direct Immunofluorescence in cutaneous Vesiculobullous lesions. Indian J Pathol Microbiol. 2007; 50(4): 730-32
Image of Alex Wood
This essay was reviewed by
Alex Wood

Cite this Essay

Vesiculobullous Disorders. (2018, September 14). GradesFixer. Retrieved November 19, 2024, from https://gradesfixer.com/free-essay-examples/vesiculobullous-disorders/
“Vesiculobullous Disorders.” GradesFixer, 14 Sept. 2018, gradesfixer.com/free-essay-examples/vesiculobullous-disorders/
Vesiculobullous Disorders. [online]. Available at: <https://gradesfixer.com/free-essay-examples/vesiculobullous-disorders/> [Accessed 19 Nov. 2024].
Vesiculobullous Disorders [Internet]. GradesFixer. 2018 Sept 14 [cited 2024 Nov 19]. Available from: https://gradesfixer.com/free-essay-examples/vesiculobullous-disorders/
copy
Keep in mind: This sample was shared by another student.
  • 450+ experts on 30 subjects ready to help
  • Custom essay delivered in as few as 3 hours
Write my essay

Still can’t find what you need?

Browse our vast selection of original essay samples, each expertly formatted and styled

close

Where do you want us to send this sample?

    By clicking “Continue”, you agree to our terms of service and privacy policy.

    close

    Be careful. This essay is not unique

    This essay was donated by a student and is likely to have been used and submitted before

    Download this Sample

    Free samples may contain mistakes and not unique parts

    close

    Sorry, we could not paraphrase this essay. Our professional writers can rewrite it and get you a unique paper.

    close

    Thanks!

    Please check your inbox.

    We can write you a custom essay that will follow your exact instructions and meet the deadlines. Let's fix your grades together!

    clock-banner-side

    Get Your
    Personalized Essay in 3 Hours or Less!

    exit-popup-close
    We can help you get a better grade and deliver your task on time!
    • Instructions Followed To The Letter
    • Deadlines Met At Every Stage
    • Unique And Plagiarism Free
    Order your paper now